Is the file on a local Windows desktop? Is it on a network drive? What is your network and desktop operating system?
Some network operating systems cannot pass login credentials to Access (NetWare most notably) so you will not be able to log in unless you move the file to a Windows only workstation and make sure the username and password on the workstation match the Access file.
I always code my own security since Access security is very funky. It is not the most robust, but works fine for most environments (not sophisticated users). Also, consider a SQL back end if security is an issue.
HTH
mmcdonal
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