I ran into similar problems when I tried to create my first web service that accessed an access database. My problem was solved by granting the proper permissions to the folder the DB was in. It was actually an IIS problem, but the point is, grant ASP/ASPNET user permissions to the database folder and see if that fixes it like it did for me. From explorer, right click to access the property page for the folder, and as I recall, it was under "Advanced". There you get a list of all the system users and something like ASP or ASPNET user should be there. Add it to the file folder permissions.
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