It's hard to determine without any information on what errors your getting, for instance.
One thing to check: Make sure the database is of the latest file format. For some reason MS in it's wisdom decided you can't make a MDE file when the format is not completely up to date. For instance, if the current format is Office 2000 and you are using Office 2003 then in order to make an MDE you have to update your current MDB to use the Office 2003 format.
BE SURE to make a COPY of your database and make your changes/updates to the copy just in case. I've seen the update fail and corrupt the MDB file beyond repair.
If this isn't the issue then more information will be needed to help you with the problem.
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